FastQCFastQC Report
Wed 8 May 2024
EGAF00007947700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10216645
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2207732.1609148600152004Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAC1002730.9814670080050741TruSeq Adapter, Index 25 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTG876220.8576396654674797TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAA675050.6607354958501543TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA666980.6528366210238292TruSeq Adapter, Index 25 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT593820.5812279862909987TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCT588170.5756977951176732TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCA579750.5674563420770713TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGC570770.5586667638936265TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCC568300.5562491404957303TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTA533660.5223436852313064TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC503980.4932930526606337TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAT501170.49054263899744TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTC487480.4771429368447274TruSeq Adapter, Index 25 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA463220.45339737262085544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431880.4227219405196128No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACT413190.4044282638772317TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTT411370.4026468571629923TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT411350.40262728126503367TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTG395020.3866435605817761TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGA345860.3385260033993547TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACC340130.3329175086341945TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTC318860.31209854115514435TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCT306040.29955039056363414TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCC295860.28958625850266895TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCC276070.27021590747256075TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACA260930.25539695271784424TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCT251610.24627458426910204TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCT248120.24285859007531335TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAC245950.2407346051467972TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTG240370.2352729296163271TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAG225840.22105103974935017TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAC225500.22071824948405272TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCA216940.21233976515774014TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTT211100.20662360295380724TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAC206260.20188623564780808TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTA205360.20100532023966772TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAA201590.1973152634744576TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCC195560.19141313023991732TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCA195300.19115864356645454TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCA190740.1866953388318768TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAT188520.18452241415846396TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTC178800.17500852775054826TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATC174640.170936740975144TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAA171060.1674326552405413TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGT170730.16710965292422317TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCG169900.16629725315893817TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAT164540.16105091250601347TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGAC160780.1573706436897827TruSeq Adapter, Index 25 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTT160090.15669527521020843TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAA156310.152995430496019TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT153940.15067568658791608TruSeq Adapter, Index 6 (96% over 33bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA151800.14858106550633793No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA149230.1460655626186483TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTA146850.14373603076156605TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC141050.1380590203535505TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATT140400.1374228036698936TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTGC139800.13683552673113336TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTT136570.13367401921080746TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT133740.13090402964965503TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATA129010.12627432978242858TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAT125960.12328900534373075TruSeq Adapter, Index 25 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTG120140.11759241903775654TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGG114700.11226777479299711TruSeq Adapter, Index 25 (97% over 37bp)
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC114670.11223841094605912No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAGC105260.10302795095650284TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)