FastQCFastQC Report
Wed 8 May 2024
EGAF00007947701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10216645
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2475842.423339560100209Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGTAGATC1034371.012436078575697Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG485290.47499937601825254No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA362150.3544705722866949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337650.3304900972873189No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGT168230.1646626656793889Illumina Single End Sequencing Primer (100% over 33bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC145050.14197419994528535No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGT133060.13023844911906013Illumina Single End Sequencing Primer (100% over 33bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA132110.1293085939660231No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTG126630.12394479792534634Illumina Single End Sequencing Primer (100% over 33bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG113330.11092682578282792No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC107260.10498554075237027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)