FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174280
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6423565.748522499883661Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAA906330.8110858149249885TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCA607230.5434175624738238TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTA584320.5229151229430442TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAA570260.5103326567796762TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAT545080.48779876645296166TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTT526400.4710818057181313TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTA507960.4545796239220782TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTA502020.44926384518734097TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACT483750.4329137984729218TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAT479260.4288956424932971TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCT466810.4177539850442265TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCA449840.40256732424818426TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAC449770.40250468039104087TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCT441680.3952648403297573TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAC408110.36522263626828755TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACA399250.3572937137784269TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAC398570.35668517345189127TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTC398000.35617507347229527TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCT387640.34690378261507676TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTT374980.33557419359457613TruSeq Adapter, Index 8 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA365320.32692934130879125No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACC362070.32402087651284917TruSeq Adapter, Index 8 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCT361990.3239492835332567TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATT361900.32386874143121525TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAC359410.32164040994140114TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAA355970.318561911818927TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTG337740.30224766159430405TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAT333040.2980415740432493TruSeq Adapter, Index 8 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG327860.29340592861464004Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTA326050.2917861374513615TruSeq Adapter, Index 8 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC323280.2893072305329739TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAA319570.28598710610437544TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAT311850.27907838357370673TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCA306630.27440694165530133TruSeq Adapter, Index 8 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTT305860.27371785922672426TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTC304440.27244708383895877TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATA299710.2682141489205569TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTT296950.26574419112461833TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTT295140.2641243999613398TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTC291310.2606968860633526TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATC289150.25876387561435726TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGA288570.2582448265123122TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCC286990.25683086516536185TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCT284790.2548620582265703TruSeq Adapter, Index 8 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT280940.251416646083685TruSeq Adapter, Index 8 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGA279190.2498505496551008TruSeq Adapter, Index 8 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT273910.24512541300200102Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGT257640.23056519077739238TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCC244650.21894028071607297TruSeq Adapter, Index 8 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAG241850.21643452643033823TruSeq Adapter, Index 8 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC235650.21088607051192562Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCC222250.19889424643019507TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTC222070.19873316222611212TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC214840.19226294669544705TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAG213380.19095637481788535TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAG209050.18708140479744556TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCG207710.18588222238927252TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAA205530.18393131369537902TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAC203530.18214148920556852TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCG200950.17983261561371292TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGC194490.17405148251162492TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCA194310.17389039830754197TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGC192820.17255697906263312TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGC186800.1671696073483034TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGA184210.16485178463399877TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAT180840.16183593036866803TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCC167720.150094681715511TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGA164660.14735625024610086TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC158190.1415661680215638Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGG149510.1337983297357861TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAG149430.13372673675619368TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTG144510.12932376851125976TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT143750.12864363520513178Illumina PCR Primer Index 2 (96% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTG143160.1281156369806377TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAG143040.12800824751124903TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTG140530.12576201777653684TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACG134900.12072366183772018TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTA133430.11940814083770945TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTT132280.1183789917560684TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATG130040.11637438832748061TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGC128790.11525574802134902TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGT127650.11423554806215702TruSeq Adapter, Index 8 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC126340.11306321302133111Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGT112040.10026596791918585TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)