FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174280
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6853226.133030495029657Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGT987630.8838421804357864Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510840.4571569711874054No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGTAGATC410770.3676031028397355Illumina Single End PCR Primer 1 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337310.3018628493289948No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT294240.26331897894092504Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG285800.2557659195939246Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTG255860.22897224698146101Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG245680.2198620403283254Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCA227060.2031987743281894Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTC197080.17636930522592953Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGT196160.1755459859606167Illumina Single End PCR Primer 1 (97% over 35bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA185660.16614940738911144Illumina Single End PCR Primer 1 (100% over 26bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG165230.14786635022569689Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG130750.11700977602136335Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTG113490.10156359067429847Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)