FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15901488
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG173690010.922877154641125Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1205120.7578661820830856No Hit
AAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG992690.6242749106247164Illumina PCR Primer Index 4 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA956420.6014657244655344Illumina Single End PCR Primer 1 (100% over 25bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA698180.43906582830487306Illumina Single End PCR Primer 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680150.42772726678157413No Hit
ATATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG557770.35076591574323107Illumina PCR Primer Index 2 (96% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA549050.34528215221116415Illumina Single End PCR Primer 1 (100% over 25bp)
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG545990.3433578039992232Illumina PCR Primer Index 4 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC504070.316995491239562Illumina Single End PCR Primer 1 (96% over 33bp)
GAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG433850.2728361018792707Illumina PCR Primer Index 2 (96% over 26bp)
ACATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG383020.24087053991425203Illumina PCR Primer Index 3 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGT332430.2090559072207582Illumina Single End PCR Primer 1 (96% over 33bp)
TTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG296920.18672466375473792Illumina PCR Primer Index 2 (96% over 26bp)
CATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA244630.1538409487212769Illumina Single End PCR Primer 1 (100% over 25bp)
GTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG241590.15192917794862973Illumina PCR Primer Index 2 (100% over 26bp)
GATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA215100.13527035960408232Illumina Single End Adapter 1 (96% over 25bp)
AGATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG204480.12859173933911092Illumina Single End Adapter 1 (96% over 26bp)
CTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG179650.11297684845594325Illumina Paired End PCR Primer 2 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG179080.11261839143607189Illumina Single End PCR Primer 1 (100% over 32bp)
TGATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG172630.10856216726384349Illumina Paired End PCR Primer 2 (96% over 26bp)
CAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG163070.10255015128143982Illumina Paired End PCR Primer 2 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)