FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18211387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG278005315.265465502435372Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3986682.189113876938643No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA480060.26360430427402376No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA429180.23566574034146878No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC349210.1917536539089527No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACA335580.18426932555988185TruSeq Adapter, Index 13 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT318780.17504432803498163Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCA277240.15223442344067478TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT263890.14490384505035228Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCT257180.14121933711034748TruSeq Adapter, Index 13 (97% over 37bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC255130.14009366776951146No Hit
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG255020.14003326600000318No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC249770.13715045427347186Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC247300.13579415999451333No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA242530.1331749196258363No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACT227270.12479554687405194TruSeq Adapter, Index 13 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAC218510.1199853695932111TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAA210900.11580666535722951TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTA205470.11282501437150286TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCA205240.11269871976253099TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG201980.11090863095710392Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTT197560.10848157803686233TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACACT193880.10646086429331274TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAT191170.10497278433542706TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACCTC186680.10250729392549836TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATC186220.10225470470755467TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)