FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8027796
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214930.2677322642478708No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT164910.20542375516268727No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG153540.19126046551257656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA105960.13199139589496295No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA89540.11153746308451287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)