FastQCFastQC Report
Thu 2 May 2024
EGAF00007947714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30071843
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG26598788.845078101797752Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2499530.8311861697335943No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGA865870.2879337990691159TruSeq Adapter, Index 25 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGT695390.23124289389246946TruSeq Adapter, Index 25 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAA557200.18528960795651933TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTA483440.16076167995423493TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAA444700.1478791971612781TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACT435870.1449428955850827TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAT411470.13682899315482594TruSeq Adapter, Index 25 (97% over 40bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTA408740.13592116718619474TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAA407350.13545894077725798TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTC403730.13425515689211331TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTT394800.1312856016174333TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAT370260.12312514400929798TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACA368220.12244676856021094TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAC366690.12193798697339568TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAC365190.1214391814961258TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTCT334300.11116711403421466TruSeq Adapter, Index 25 (97% over 40bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGC333270.11082460093982267TruSeq Adapter, Index 25 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGG324430.10788497399377883TruSeq Adapter, Index 25 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCCA322350.10719329706529793TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGATA313600.10428359844789027TruSeq Adapter, Index 25 (97% over 40bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCA311360.1035387156018339TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCCT304100.10112449709184768TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTAA303610.10096155396927287TruSeq Adapter, Index 25 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)