FastQCFastQC Report
Fri 3 May 2024
EGAF00007947716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34673435
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG26380627.608308781636432Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10480213.0225473766876574No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGGA812950.23445903182075845TruSeq Adapter, Index 16 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382540.11032653672761294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)