FastQCFastQC Report
Thu 2 May 2024
EGAF00007947720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29277338
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8845183.0211694792743793No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5868012.0042839960381644Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443350.15143111713230212No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413270.14115695901041275No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409610.13990684535595416No Hit
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377270.128860759130492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)