FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29308369
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG447927115.283248958684803Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6888272.3502740804170985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA931310.31776247937918345No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGT926840.3162373177436111Illumina Single End PCR Primer 1 (96% over 33bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC803110.27402070719117805No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA799660.2728435690160718Illumina Single End PCR Primer 1 (100% over 26bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC716960.24462637276062685No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC669400.22839892591771313Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA609540.20797472558094243No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT579240.19763638160827032Illumina Single End PCR Primer 1 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA526360.1795937535793957Illumina Single End PCR Primer 1 (100% over 25bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA504630.17217948907358166No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA500350.17071915533750787No Hit
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC477670.1629807513342008No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTG406490.1386941729851975Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT387870.13234103883433432No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG384710.13126284850583123Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT373210.12733905458881045Illumina Single End PCR Primer 1 (100% over 31bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA354370.1209108565543173Illumina Single End PCR Primer 1 (100% over 25bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG324420.11069193239651104No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG314500.10730723364374183No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT305030.10407607465294298No Hit
TCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCG295520.10083126768330233Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)