FastQCFastQC Report
Wed 15 May 2024
EGAF00007947726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43934459
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG8509911.9369556820991014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3051700.6946028401078069No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGAA479940.10923999314524392TruSeq Adapter, Index 2 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGAC443670.10098451422834182TruSeq Adapter, Index 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)