FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27469271
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG459047916.711324446870105Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGT1397910.5088995627150061TruSeq Adapter, Index 6 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA738010.26866748666173196No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAA682450.24844124913253068TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTA667390.2429587592623044TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG665640.24232168374617585TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTA570330.2076247309220547TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAT557520.2029613381439937TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTT541280.19704927735432076TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGC533150.19408960652796356TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAC506860.18451891205995238TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTT471120.17150800980484704TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTC456780.16628763100411365TruSeq Adapter, Index 3 (96% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTA455610.1658617005161877TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCT445980.16235596496172033TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTT418580.1523811825949076TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC417120.15184967959288037Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTT407220.14824565238735313TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTC400200.14569006945979746TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG399490.1454315988218253Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGA389100.1416491904717821TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGT381250.13879145172800547TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAG380450.13850021720634667TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTG376200.13695303381003449TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTT371530.13525295228985146TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAA344650.12546747236211692TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTG340330.12389480594515959TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAC329760.12004686982774314TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAT321450.11702167123401272TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGCT320690.1167449984384369TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAA319930.11646832564286107TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTG319340.11625354018313773TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTACT318150.1158203288321703TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAC314440.11446972873797781TruSeq Adapter, Index 6 (97% over 36bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC313420.11409840472286287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAT307400.11190686494738065TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCA296250.1078477838017616TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG293450.10682846297595593Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTTA280240.10201945293706557TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)