FastQCFastQC Report
Wed 1 May 2024
EGAF00007947733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27469271
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG435402215.85051893077177Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3334401.2138654862737348No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT880060.3203798164137665Illumina Single End PCR Primer 1 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGTAGATC547480.1993063448971762Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGT505150.18389639826990675Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT489730.17828285286493406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA468530.17056513804097678No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG389140.14166375219786503Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGAC386560.1407245208655155Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT346590.12617371607713945Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGT338550.12324680913446884Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTG324970.1183031031293113Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)