FastQCFastQC Report
Thu 2 May 2024
EGAF00007947735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43812070
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1509355534.45067763289888Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8303811.8953247358547543No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGT3567640.8143052816267298Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGT2317470.5289569746419195Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT1952990.4457652879674482Illumina Single End PCR Primer 1 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC1903840.43454691823508906Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1797040.41017007413710427Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTG1743460.3979405675194074Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAT1342180.30634936902091137Illumina Single End PCR Primer 1 (97% over 35bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGA1281800.29256777869660117Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1250230.2853620018410452Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT1119210.2554570007762701No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG973090.2221054608923979Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGT894510.20416976417685812Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT879230.20068214078905652Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT842800.19236708057847987Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG800910.1828057884505343Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG743140.16961992437243892Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG721700.16472629574452885Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG652360.14889960688915177Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT643580.14689559292678936No Hit
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATG599850.13691432520764255Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG576850.1316646303176271Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT574180.13105520921517747No Hit
CGTATCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT542660.12386084473981712Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT517350.11808389788476098Illumina Single End PCR Primer 1 (100% over 31bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA484820.11065900332944781Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATGCCGTCTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT481960.11000621518225458Illumina Single End PCR Primer 1 (100% over 31bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG467050.10660304340790107Illumina Single End PCR Primer 1 (100% over 28bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTA447350.10210656561080086Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTGGGAAAGAGT441120.1006845830384184Illumina Single End PCR Primer 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)