FastQCFastQC Report
Mon 3 Apr 2023
EGAF00007947736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51951178
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG607347711.690739717201408Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCG2503930.4819775212796907TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1636440.31499574465857155No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCT1499430.2886229066836559TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCA1489520.2867153464739529TruSeq Adapter, Index 7 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACA1275790.2455747971682182TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTA1200030.23099187471745108TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCC1191910.2294288687736782TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTG1162920.2238486295729425TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTC1142050.2198313963159796TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAA1112290.21410294103436883TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGC1112060.21405866869852308TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAC1101880.2120991366163054TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAC1068490.20567194838199818TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAC1051430.2023880959927415TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTA1050930.2022918517843811TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGC1020560.19644597856857066TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAA996620.19183780587227492TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTT991150.1907848942328122TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGC980070.18865212257554584TruSeq Adapter, Index 7 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGG967500.18623254317736548TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAT946350.18216141316372075TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA939790.18089868915003238TruSeq Adapter, Index 10 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAT921710.17741849857572045TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC913470.17583239402194115Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTC879950.16938018229346022TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCC876030.1686256276999147TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT865400.1665794758301727TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAC861260.16578257378494862TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCA817190.15729960926006337TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTT814830.1568453365966023TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACT787550.1515942525884591TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGT773370.1488647668393583TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCT756020.14552509280925258TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG744360.14328067787028814Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCT732430.1409842910588091TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCT710450.13675339565928613TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTA707140.1361162589999403TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACC690800.13297099827072256TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT677680.13044555024334578TruSeq Adapter, Index 10 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACA674490.12983151219400646TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAT654180.12592207245040718TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCG651400.1253869546519234TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCC650510.1252156399610419TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAGC647260.12459005260669932TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAG645980.1243436674332967TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAA639950.12318296228047032TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTT629610.12119263205157735TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAA625550.12041112907969094TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGA624230.12015704436961949TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT612640.11792610361982551Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCC604240.11630920091937089TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGT603660.11619755763767282TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTC593900.11431887069047789TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA591760.11390694547869538TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATG588310.11324286044100866TruSeq Adapter, Index 7 (97% over 35bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAT583760.11236703814492907TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAT582300.11208600505651671TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCA573120.11031896139101986TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATA560310.10785318477282652TruSeq Adapter, Index 7 (97% over 35bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT548640.10560684494969488Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATC548200.10552215004633773TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCGC533180.10263097402719146TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)