FastQCFastQC Report
Tue 14 May 2024
EGAF00007947737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51951178
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG604763211.64099108589992Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4683600.9015387485534977No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG2465600.4745994402667828Illumina Single End Sequencing Primer (100% over 33bp)
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG2116650.4074306072520627Illumina Single End Sequencing Primer (100% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGT1593490.3067283671604136Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAGATC1333520.2566871534655095Illumina Single End Sequencing Primer (100% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1221610.23514577475028572No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG903030.17382281495137608Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCAC828310.15944008045399855Illumina Single End Sequencing Primer (100% over 33bp)
CGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG772740.1487434991368242Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTG725550.13965997075177006Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT687170.13227226531802608Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTT673120.12956780306309898Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT626450.12058436865473965Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)