FastQCFastQC Report
Fri 3 May 2024
EGAF00007947738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419376
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG811574017.483518089515034Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGT4380480.9436748998952506TruSeq Adapter, Index 9 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTA1835130.3953370678657981TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAA1792590.38617279129301524TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACT1782720.3840465240204866TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACTC1661900.358018599819179TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACA1596990.3440352149498951TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAC1525290.3285890788363894TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAC1506280.32449380620713214TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAA1436840.3095345357507606TruSeq Adapter, Index 9 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTC1400390.30168221132485706TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAACT1320590.28449111422781725TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACAC1309330.2820654030334229TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATA1305700.28128340200006136TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAAA1287490.27736047119633833TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACTA1271370.27388778341182357TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAT1271280.27386839495645093TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACC1234620.2659708308013447TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACAT1185470.2553825798950852TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAAC1167450.25150058027492656TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACCC1161390.2501950909465048TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG1145720.24681934543885295Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAACA1120770.24144443475500404TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAA1114550.24010447706147536TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACTT1110790.23929447048146446TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATAA1109810.23908335174518502TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAAT1097760.2364874529980756TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAT1053240.22689663040709554TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAACC1040570.2241671667451971TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATCT1029150.2217069871856959TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACAA1020290.21979830146790427TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGC1013790.2183980241354386TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATGC996270.2146237381562389TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACCA990760.21343673383287187TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAATA985970.21240483715248565TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATC956940.20615098315841215TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACCT950710.20480887119206426TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATT931120.20058865073929472TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAATC919300.19804230026702643TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATAC897990.19345154488935826TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGCT892030.19216759828912824TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAT880560.18969664736553116TruSeq Adapter, Index 6 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATG878440.1892399415278654TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAG874710.18843639776631205TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATTA856580.1845307011451425TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATAT856460.18450484987131235TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAG847990.18268018079346868TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAATT833950.17965558175534285TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATTC827730.1783156240618142TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGCA808770.17423112279665284TruSeq Adapter, Index 9 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC805330.17349005294685563Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGCC789530.17008630189255453TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATCA773630.16666100811006163TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAC753170.16225336592202358TruSeq Adapter, Index 6 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG737110.15879360377442386Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAGC706330.1521627520369942TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGA702270.15128811727240796TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATTT675150.14544572938679745TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGAA662770.1427787396366552TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAAG659590.1420936808801566TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATCC637870.13741460031690214TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACTG629090.1355231487816639TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATGA625540.13475838193085576TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATGT623380.13429305900191335TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAATG619820.133526137878286TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAG615120.13251362965327237TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAGT611220.13167346325379298TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGAC603130.12993065654307803TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAAGA589550.12700515405463444TruSeq Adapter, Index 9 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT584070.12582461254972493Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG580960.1251546337029606Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGT576870.12427353611991683TruSeq Adapter, Index 9 (97% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCT570710.12294650406330322TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTACG569510.12268799132500187TruSeq Adapter, Index 9 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTG530700.11432725851377235TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTATAG510010.10987006805089324TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGTA492230.10603977097839488TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCTGTAGTC485660.104624413736195TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)