FastQCFastQC Report
Sun 5 May 2024
EGAF00007947739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419376
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG782551716.85829856911476Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6663011.4353941336910692No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGT1649330.3553106788854723Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGTAGATC1248830.26903205247739653Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT1010670.2177258910158551Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGC804370.17328324275621454Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG747730.16108144150839082Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAC740090.15943557707453887Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTG640370.13795316852169665Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT563610.12141697036168689Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT497530.10718153557255919Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT486740.1048570752006662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)