FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25439403
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG8451783.3223185308240137Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT979280.3849461404420536Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAA899960.35376616345910317TruSeq Adapter, Index 3 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA857120.33692614563321316No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAT663630.2608669708168859TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAA607190.23868091558595148TruSeq Adapter, Index 3 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT544370.21398693986647405TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATA513460.20183649749956786TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATT481120.18912393502316072TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT458210.1801182205415748TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAA434510.17080196418131352TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAT419300.16482305028934838TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTA399850.1571774306181635TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGA382030.1501725492536126TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACA375660.14766855967492634TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAA365100.1435175188663036TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAG354870.1394961980829503TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAC328800.1292483160866629TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTGA314490.12362318408179625TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATC305040.1199084742672617TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCA303080.11913801593535824TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTA298610.11738089922943554TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAG296650.11661044089753207TruSeq Adapter, Index 3 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA295210.11604438987817443TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGT294550.11578494982763549TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCT293920.11553730250666652TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC292410.11494373511831232RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAT292250.11488084056060592TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATG290650.11425189498354188TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCGAA283930.11161032355987285TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACT280810.11038387968459794TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTT278600.10951514860627821TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA275210.10818257016487376TruSeq Adapter, Index 3 (96% over 33bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTC260910.1025613690698638TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)