FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25439403
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG8503803.3427671238983083Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1528000.6006430260961706No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA1451070.5704025365689596Illumina Single End PCR Primer 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1001930.3938496512673666No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTAACTGTGT596500.23447877294919223Illumina Single End PCR Primer 1 (96% over 33bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA442310.1738680738695008Illumina Single End PCR Primer 1 (100% over 25bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA313830.12336374403125734Illumina Single End PCR Primer 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)