FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007947744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15841008
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG237101514.967576558259424Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCG607190.3833026282165882TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500100.3156996069947064No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAA451930.2852911885405272TruSeq Adapter, Index 2 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGT311970.19693822514324846TruSeq Adapter, Index 2 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA296030.18687573417045178Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC226460.1429580743851654Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC217380.1372261159138358Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAC215850.13626026828595755TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAA204040.12880493463547268TruSeq Adapter, Index 2 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT192060.12124228458189025Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTA188110.11874875639226999TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAT184270.11632466822818345TruSeq Adapter, Index 2 (97% over 36bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG183010.11552926429934257TruSeq Adapter, Index 2 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG179760.11347762718130058Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAG177290.11191838297158868TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACA174870.11039070240984664TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAAA160120.10107942625873303TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)