FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15841008
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG225642514.244200874085791Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1036260.6541629169052878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA333260.21037802644882195No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCAGGTGTGT298840.18864961118635887Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT292690.18476728248606403Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCA265100.16735046153628608Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG217660.1374028723424671Illumina Single End PCR Primer 1 (100% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG208420.1315699101976339Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCAGGT189430.11958203669867475Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT178860.1129094815178428Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG174360.1100687532005539Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA173420.10947535661872024Illumina Single End PCR Primer 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)