FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8070808
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5078936.292963480236427Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA785370.973099595480403No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAAC493600.6115868448363535TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAA438590.5434276221166456TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTA391190.48469744293260353TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACT342720.4246414981003141TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACG326290.4042841807164785TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAC301300.3733207381466639TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTA294080.3643749176042845TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAT285000.3531244950939237TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGA269160.33349820736659824TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGC255300.31632520560518845TruSeq Adapter, Index 6 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAA246490.3054093220901798TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTT212200.26292286968045825TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCA206440.2557860377796126TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT202750.25121400484313344Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAG196490.2434576562842283TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTG192790.23887323301458788TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGT182110.22564035719843661TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC178460.2211178855946022Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTT173830.21538116134097104TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTC167230.20720354145458544TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCT149660.18543372609037412TruSeq Adapter, Index 6 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGA131810.1633169813976494TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTG127870.15843519013214044TruSeq Adapter, Index 6 (97% over 36bp)
CCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAG120030.14872116893376722No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTA119510.14807687160938532TruSeq Adapter, Index 9 (96% over 33bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAC112760.13971339672558186TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTC106790.13231636782835127TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT102660.12719916023277966Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG102520.127025695568523Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGCC102240.12667876624000968TruSeq Adapter, Index 6 (97% over 36bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA98320.12182175564082307TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG95870.11878612401633144Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCC95830.11873656268368668TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGCT95400.1182037783577555TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTT93780.11619654438564267TruSeq Adapter, Index 9 (96% over 33bp)
CTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGG93040.1152796597317146No Hit
CCCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGC88590.10976596147498491No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCTC86850.1076100435049378TruSeq Adapter, Index 6 (97% over 36bp)
CAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT86810.10756048217229304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCGGGCTCCGGCG83010.10285215557104073No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAAA82770.10255478757517215TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAAC82020.10162551258808288TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)