FastQCFastQC Report
Wed 8 May 2024
EGAF00007947747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8070808
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5414006.708126373468431Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA687880.8523062374919587No Hit
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC398850.4941884381340753No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC344530.42688414840248956No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG298980.37044618085326775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239570.29683521154263615No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCA224270.27787800180601496Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT216080.2677303189470001Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG184950.2291592118162147Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGC145770.1806138864906711No Hit
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG132490.16415952405261033No Hit
CTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC131340.16273463573907346No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGC131320.1627098550727511No Hit
CCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCC124020.15366491186508216No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCC120740.14960088258821172No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG119260.14776711328035558Illumina Single End PCR Primer 1 (96% over 30bp)
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG114400.14174541136401708No Hit
TCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCC113350.1404444263820921No Hit
CGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCC107690.13343149781285837No Hit
GCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCG104890.1299622045277251No Hit
CGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGCGCCGAGG103290.12797975122193467No Hit
CCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCCTCCGG102720.1272735022317468No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA101540.12581144291872634No Hit
CGGTCCCGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCC100680.12474587426686398No Hit
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC91510.11338393875805247No Hit
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT91270.11308657076218391Illumina Single End PCR Primer 1 (100% over 31bp)
CCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCC90750.11244227343780201No Hit
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC90360.1119590504445156Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCATTG90290.11187231811238726Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT89470.11085631079316965Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAT88090.1091464448169254Illumina Single End Sequencing Primer (100% over 33bp)
CCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGA88050.10909688348428064No Hit
GGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGCGC85560.10601169052714425No Hit
CCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGG84970.10528066087063402No Hit
CCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGA83190.10307518156794215No Hit
CCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGG81160.1005599439362205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)