FastQCFastQC Report
Mon 13 May 2024
EGAF00007947749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66838544
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG13706352.0506655560899114Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11620411.7385791647406323No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840520.275368057089933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1710690.2559436363544963No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1358490.20324949029410339No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA888750.13296968288237995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)