FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13774271
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG287942620.904380347969052Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGC989940.7186877621327473TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT836360.6071900284232828TruSeq Adapter, Index 10 (96% over 33bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATC632950.4595161515262768TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTA621820.4514358690924551TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTT615470.44682582475689636TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGT612580.44472771009079176TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA555880.4035640071260395TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTG520810.3781034945515447TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCT484380.35165563389888294TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCA476730.3461018009591942TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAA422670.30685471485206006TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC409830.29753298740819023TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGA395090.28683187661982257TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAT386340.2804794533227929TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG381200.2767478583803092TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAT378980.2751361578409485TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTC367320.2666711000531353TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGC364910.26492146117932486TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTA359460.2609648089543178TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAG353380.2565507822519246TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGG344020.24975550430218776TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCG337470.24500026171983985TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTT335620.24365717793703925TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTT329590.2392794507963434TruSeq Adapter, Index 9 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG308650.224077194357509TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAA308340.2238521370749857TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT305920.22209523828883576TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG303920.22064325582094327Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGT300000.21779737018387396TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTA299260.21726013667075378TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAT296400.21518380174166749TruSeq Adapter, Index 9 (97% over 39bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCA284580.20660258535642284TruSeq Adapter, Index 9 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTA275910.20030824135810887TruSeq Adapter, Index 9 (97% over 39bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC274920.1995895100365021Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTG271920.19741153633466338TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGA268560.19497220578860397TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTG262660.19068885750832112TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAC262330.19044928040111886TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAA258650.18777763266019667TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTT247020.17933435460940184TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCC244920.1778097730181147TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTC243160.1765320284463693TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG241550.17536318255971586Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCT241220.17512360545251363TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAT232980.16914143768479653TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG227620.16525012467084466TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGG227300.16501780747598185TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAC224840.16323186904047407TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCT218170.15838950751005262TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGC216470.15715532241234398TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTC216360.15707546337660994TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAT216170.15693752504216013TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAG213290.15484667028839494TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGT212510.15428039712591687TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGC211620.1536342649277047TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAA205290.14903874041682497TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATG204500.1484652073420074TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAA204200.14824740997182356TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC203480.14772469628338225TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCA196270.1424902994866298TruSeq Adapter, Index 9 (97% over 39bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC195460.14190224658713335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGT192340.13963715393722106TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCA191380.13894020235263269TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGT187810.13634841364744457TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAC184180.13371306546821968TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTC183980.13356786722143044TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATT176610.12821731182724663TruSeq Adapter, Index 9 (97% over 39bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA174260.12651123242747295TruSeq Adapter, Index 7 (97% over 35bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT172890.1255166244369666TruSeq Adapter, Index 9 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAC171550.12454379618347859TruSeq Adapter, Index 9 (97% over 39bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT170480.12376698556315611Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA170380.1236943864397615TruSeq Adapter, Index 9 (97% over 39bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT165160.11990471219856208Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAG163130.1184309499936512TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAG161170.11700800717511656TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATA160190.11629653576584924TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAC159540.11582464146378418TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG159420.11573752251571062TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGC157150.11408952241465264TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTG154680.11229632406680543TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCC152370.1106192843163896TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG150990.10961741641354378Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAG149040.10820173350734859TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGG145320.10550104611706855TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGG144110.1046225967239936TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCC142160.10320691381779841TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGC141490.10272049969105443TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCCA139380.10118865818742784TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCCC138320.10041910747944484TruSeq Adapter, Index 9 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)