FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13774271
Sequences flagged as poor quality0
Sequence length151
%GC68

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG277235120.127025234221108Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6693254.859240826610715No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGT1001500.7270802207971659Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTG630770.4579334906362739Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC587360.4264182111706674Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT508640.36926818123441885Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGT506490.3677073000814345Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTA378170.2745481049414521Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT374430.2718328977264931No Hit
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGA363640.2639994523122131Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG257250.18676124493267193Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGT211530.15356892571664954Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT188050.13652265154359167Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTAT163730.11886654473401896Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT160390.1164417340126385Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG154040.11183168967707983Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTA138850.10080388283343633Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)