FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23290873
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG453384119.46617028910853Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1203310.5166444383600391No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGA1158340.49733644591166676TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGT959880.4121271023202952TruSeq Adapter, Index 7 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTA584480.2509480859734197TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG549510.23593362086513459Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGC546540.23465844324512866TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGG519040.22285124305988874TruSeq Adapter, Index 7 (96% over 33bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT349940.1502476957390133Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC333580.14322348501063056TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG333470.14317625620988958Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT270670.11621290451414165Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC262090.11252905805634679Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCT260170.11170470080705003TruSeq Adapter, Index 23 (97% over 37bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA255910.10987565816017288No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCA246450.10581398129645032TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)