FastQCFastQC Report
Wed 1 May 2024
EGAF00007947758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25543630
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG7977883.1232365955817554Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5933602.322927477418049No Hit
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT405780.15885760950968988Illumina PCR Primer Index 2 (96% over 31bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC288420.11291269095269545No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA279730.10951066860896434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)