FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22887338
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG435130319.011835277654395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1026510.44850563224084866No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCT603530.26369602266545805TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAA538820.23542274772190633TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG535200.2338410871548277Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT499080.21805943530872834Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA455860.19917563152167367TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACT435520.19028862159504964TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAT421600.1842066560995429TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGC403470.17628524557989225TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATA380260.1661442671926285TruSeq Adapter, Index 1 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA375590.16410383767653539TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATT373950.16338728427045557TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCT371210.16219011577493198TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTT367890.16073953205042893TruSeq Adapter, Index 1 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT365790.1598219941523999Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTA353190.1543167667642257TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG346390.151345691665846Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAC340930.1489600931309705TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAT338300.14781098614439128TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTT336940.14721677112471535TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAT330790.14452969585191602TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT329620.14401849616587126TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAC324470.14176834370165722TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTA324340.14171154373654113TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAT321490.14046631373207316TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGT320300.1399463755898567TruSeq Adapter, Index 1 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC304780.13316533360061358Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTA304650.1331085336354975TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATC303750.13271530310777077TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA303710.13269782619542736TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACA298820.13056127366144546TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATA297650.13005007397540072TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAA295980.12932041288506335TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATT294820.12881358242710444TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGCT294070.1284858903206655TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAA293040.1280358598278227TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACC289310.1264061377517997TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAA285670.12481573872854938TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTC284630.12436133900762072TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATG281870.12315543205592543TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCT273040.11929740365611763TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTC272950.11925808060334496TruSeq Adapter, Index 1 (97% over 37bp)
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA269460.11773322000138242No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACA268240.11720017417490843TruSeq Adapter, Index 1 (97% over 37bp)
GGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGCCAACCGAGCTTTGA260440.11379217626794344No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATG253740.11086479345042224TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACT249240.1088986408117886TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT244600.10687131897995303TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAC241620.10556928901036895TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAG237000.10355070563470509TruSeq Adapter, Index 12 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAC236160.10318369047549347TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTG234310.10237538327961075TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCA229670.10034806144777518TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)