FastQCFastQC Report
Wed 1 May 2024
EGAF00007947763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22887338
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG446267619.49844931726005Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2291651.0012741542943964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA993770.4342007794877674No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA711550.3108924244488372Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGT669250.29241058964568095Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGTAGATC566510.24752114029163197Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT490670.21438491448852637Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT376740.16460629890640843Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG307980.1345634865880864Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT272290.11896971154967868No Hit
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA251500.10988608635919127Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTAC230370.10065390741378487Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)