FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47379100
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG591739812.489468985269877Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3375990.7125483599308556No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAA1005460.21221593487423782TruSeq Adapter, Index 3 (96% over 32bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACG907180.19147261134128762TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAT897660.18946328655462008TruSeq Adapter, Index 3 (96% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG773910.16334417496322218Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAG730950.1542768857998569TruSeq Adapter, Index 3 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT673270.14210274150416535Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAG627410.13242336810956729TruSeq Adapter, Index 3 (96% over 32bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC515330.10876736789006124Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG494810.10443634429526942Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)