FastQCFastQC Report
Thu 2 May 2024
EGAF00007947768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32581631
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG9541512.928493665648598Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2159990.6629471679916822No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA390240.11977300952183763No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)