FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27347119
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG390669114.285566973252283Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1972320.7212167395037116No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTT1001230.366119005076915TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGC528280.19317574183957TruSeq Adapter, Index 7 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTG457150.16716568937298293TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCT456500.16692800437223387TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAA382540.13988310797930853TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG371340.13578761258178604Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAC366820.13413478765350018TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCC360160.131699430568902TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGA353710.12934086402300732TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAT350740.12825482640420002TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCA344900.12611931808977758TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC329990.12066718984182574Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGT325920.11917891606790462TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGTA288220.10539318602445838TruSeq Adapter, Index 7 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACG281130.10280059117013386TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)