FastQCFastQC Report
Wed 1 May 2024
EGAF00007947771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27347119
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG378810913.85194908465495Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1856990.6790441069861874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1586790.5802402805209572No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT431600.15782284049738476Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTCGCAGTGT391570.14318510114356106Illumina Single End PCR Primer 1 (97% over 38bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG306890.1122202305844356Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTCGCA296880.10855988157289988Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)