FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888789
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2852223.2087835587052407Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA917211.0318728456711033No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACA703280.7911988910975387TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTA580870.6534860935499762TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTT545170.6133231422188107TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAT542130.6099031037861289TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTC496490.5585575267902073TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAC475670.5351347635769057TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAA452050.5085619649650813TruSeq Adapter, Index 15 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACAC425230.47838912589780225TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGC415250.4671614997273532TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGT413130.46477647292561447TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTG412250.4637864618003645TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCT403970.4544713571218757TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACT391080.43996994416224755TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCA381530.4292260734280001TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAG361100.4062420651452071TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACA335490.37743049137514684TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCC299140.336536281826467TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGA277580.3122810092578415TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACG269410.303089655970009TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACA238610.2684392665862583TruSeq Adapter, Index 13 (96% over 33bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACC229410.2580891502768262TruSeq Adapter, Index 15 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGA228660.2572453907950791TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGG225850.254084105270133TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCG207780.23375512682323765TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACT188140.21165987852788498TruSeq Adapter, Index 13 (96% over 33bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAA178880.20124226145991314TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTCT171600.1930521694237539TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACC153240.17239693731058303TruSeq Adapter, Index 13 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTGC135230.1521354596222275TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGCT132310.14885042270662519TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCTC131450.14788291183422175TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTTT130490.14680289969758537TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACACT116330.13087272068219866TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACG110280.12406639419610477TruSeq Adapter, Index 13 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTCA107840.12132136334882064TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTAC107010.12038760285568709TruSeq Adapter, Index 15 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAAC106460.11976884590240583TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCCT101230.11388502978302219TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTTC97900.11013873768406474TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCTT94640.10647119647007033TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG89390.1005648800978401Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)