FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888789
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3475143.9095764338651757Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1214831.3666991082812294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG985971.1092287149576843No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCC275060.309445977399171Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACAT178970.20134351259772282Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATT134730.1515729533010627Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCCG134630.15146045203682976Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCCGT132820.14942417915421324Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCCGTG125970.1417178425542557Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)