FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8178040
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4687515.731825718631848Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCC1042081.2742417498569338TruSeq Adapter, Index 12 (97% over 34bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCA915951.1200116409310787TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAA903411.1046778934805894TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTA900111.1006426967830922TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAT859731.051266562648263TruSeq Adapter, Index 22 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAG838741.0256002660784247TruSeq Adapter, Index 22 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA747600.9141554700148201No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCT695900.8509373884206974TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTC596570.7294779678260316TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAC591140.7228382350783318TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTT535430.6547167781033109TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCG468900.5733647671080112TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCA456040.5576397278565525TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCA437050.5344190050427731TruSeq Adapter, Index 22 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGC431010.5270333722994752TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCT379540.4640965316872992TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGT352310.43080004499855706TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCT343180.41963600080214813TruSeq Adapter, Index 22 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACA327440.4003893353419646TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCC299640.3663958601327458TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGC299140.365784466693731TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAG289320.3537766995514818TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGC284690.3481151963062054TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCT280970.34356642911993585TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGA271520.3320110931225575TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCC265480.3246254603792596TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTG243600.29787088348797514TruSeq Adapter, Index 22 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTA218580.26727675579967813TruSeq Adapter, Index 22 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCT203890.2493140165614255TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCG201740.24668502477366216TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCA200220.24482638871905738TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTT199310.24371365266005057TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAC197110.24102352152838577TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACT195660.23925048055524306TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCG189520.23174256912414223TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATC186560.22812311996517504TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTC185080.22631339538569145TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCT178890.21874434461068912TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCC176960.21638436593609225TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAT166470.2035573315855633TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTGC159750.19534020376520536TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATT159090.19453316442570592TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTC158870.19426415131253943TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTT154810.18929963658773985TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGG154290.18866378741116452TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTA152590.18658504971851447TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAA152030.18590028906681796TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATA148140.1811436481112834TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAA146600.17926055631911803TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAT145840.17833123829181563TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCG143000.174858523558212TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTC141330.17281646947190282TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCC139660.17077441538559363TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAC139500.17057876948510892TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG139300.170334212109503Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACA136460.16686149737589936TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTT127740.1561987957994825TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCA126270.15440129908877923TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACC125680.15367985483074184TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCA121370.1484096433864349TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTG121160.1481528581420487TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGGC120190.14696675487036015TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTA118840.14531599258502037TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCGC116910.1429560139104235TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCC115570.14131747949386406TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAT113140.13834610738025246TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTGT109700.13413972051983114TruSeq Adapter, Index 22 (97% over 38bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAC109180.13350387134325584TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAA108680.1328924779042411TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAC108050.13212212217108255TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAGC108040.13210989430230227TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCGT104220.12743884842822975TruSeq Adapter, Index 22 (97% over 38bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG93720.11459958620892048No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTA91280.11161598622652862TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTG88110.10773975182317523TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAG86250.10546536823004046TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGAT85860.10498848134760898TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTG84290.10306870594910271TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATG84230.10299533873642096TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)