FastQCFastQC Report
Thu 30 Mar 2023
EGAF00007947777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8178040
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5590686.83621014326171Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2080172.5436045800705305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1068741.3068412480251992No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG168760.20635751353625076No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCA158260.19351825131694148Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCA131390.16066196790429005Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAGTGT107300.13120503201256045Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAGTG105380.12885728120674392Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG89210.10908481738900763Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAG82610.10101442399401324Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)