FastQCFastQC Report
Thu 2 May 2024
EGAF00007947778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38246539
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG36742359.606712387753568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2423370.6336181164000224No Hit
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC1464880.3830098195290298RNA PCR Primer, Index 29 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTTAGATCGGAAGAGCACACGTCTGAA1388680.3630864481620154Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT1026910.26849749725066624Illumina PCR Primer Index 2 (96% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAA986400.2579056891919031TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATATAGATCGGAAGAGCACACGTCTGAA967530.25297190943211884Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAAGATCGGAAGAGCACACGTCTGAA755250.1974688480962944RNA PCR Primer, Index 32 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG750420.19620598873011752Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAGATCGGAAGAGCACACGTCTGAAC731090.19115193664974495Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA600180.1569240029797206TruSeq Adapter, Index 4 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAT551170.1441097716057393TruSeq Adapter, Index 4 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCT533100.139385161099152TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCT530790.13878118488054567TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTA522130.13651692771468812TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAA520700.13614303767459848TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTT482180.12607153813316285TruSeq Adapter, Index 4 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGA460380.12037167598354455TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAT451550.11806297035138265TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAC434780.11367825988124049TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGC419230.10961253252222376TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAAAGATCGGAAGAGCACACGTCTGAA416760.10896672245297803Illumina PCR Primer Index 2 (96% over 27bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTAA414170.10828953699575275TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAG410420.10730905612139179TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA407670.1065900368135271TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATTCAGATCGGAAGAGCACACGTCTGAA406320.10623706369875716RNA PCR Primer, Index 40 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTAGATCGGAAGAGCACACGTCTGAA399950.10457155352017602RNA PCR Primer, Index 39 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTACT399330.10440944734894834TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAC396940.10378455420502232TruSeq Adapter, Index 4 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC396200.10359107264581509Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTG394780.10321979722139041TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTATA391960.10248247560387098TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)