FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38246539
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG414718610.843297481113257Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3176840.8306215629079535No Hit
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA2193500.573515946109529Illumina Single End PCR Primer 1 (100% over 25bp)
AAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG2138180.5590518922509563Illumina PCR Primer Index 4 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1898050.49626712628821135No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA1562280.40847617610576475Illumina Single End PCR Primer 1 (100% over 26bp)
ATATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG1378240.36035678940779453Illumina PCR Primer Index 2 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC1321430.3455031578151424Illumina Single End PCR Primer 1 (96% over 33bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA1091470.2853774559836643Illumina Single End PCR Primer 1 (100% over 25bp)
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG1077230.28165424327675764Illumina PCR Primer Index 4 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGT815310.2131722297800593Illumina Single End PCR Primer 1 (96% over 33bp)
GAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG712320.1862443030466103Illumina PCR Primer Index 2 (96% over 26bp)
ACATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG645840.16886233810593948Illumina PCR Primer Index 3 (96% over 26bp)
TTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG599060.1566311660252448Illumina PCR Primer Index 2 (96% over 26bp)
GTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG442960.11581701549517984Illumina PCR Primer Index 2 (100% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG423020.11060347185924456Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG421440.1101903625841805Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)