FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17344053
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG10967246.323343223178574Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5539063.193636458560176No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGGA268640.15488882558188677TruSeq Adapter, Index 16 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209060.12053699328524883No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG186780.10769109158049736No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG179520.10350521876288087Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)