FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17624327
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG309537017.563053613337974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1158070.6570860833437782No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTA966690.5484975397925832TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA591750.3357575015488535TruSeq Adapter, Index 9 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG563960.3199895235715951Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC449080.25480689276816076TruSeq Adapter, Index 9 (100% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTC432860.245603704470531TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAC426470.24197803411160043TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTA391080.2218978347371789TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCT374880.21270599439059432TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT374680.2125925148801427TruSeq Adapter, Index 9 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGC374100.21226342429983286TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAT359080.20374111306491308TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTT358240.2032644991210161TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAA345930.19627983525271633TruSeq Adapter, Index 9 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTAC341560.19380030794934752TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCT327140.1856184352457827TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCC322950.1832410395018204TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC309740.17574571783648818TruSeq Adapter, Index 9 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC299750.17007741628942769Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCA295840.1678588918600977TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT292260.16582760862301296TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCT290890.16505027397641908TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTG277800.15762304001735783TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTGC274500.15575062809490542TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACG272630.15468959467218238TruSeq Adapter, Index 9 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCTC263240.14936173165647687TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG261360.14829502425823127Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA255780.1451289459166299TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACACT253970.14410195634704234TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGT253070.14359129855000988TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCA240440.13642506746498745TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCCT230150.13058654665224947TruSeq Adapter, Index 9 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT229360.13013830258596543Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAG226410.12846447980680342TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTTT223200.12664313366405425TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACATC217760.12355649097976905TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCA215220.12211530119703294TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACACC213700.12125285691760032TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG211610.12006699603338046Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC210990.11971520955098029TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTAC209230.11871658985900567TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTTC207450.11770662221598589TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCC203330.11536894430068166TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCC202860.11510226745112026TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTAC201480.11431925882900379TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACG199000.11291211289940319TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCCC193360.10971199070466633TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG190710.10820838719118184Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG190460.10806653780311726Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCAC189940.10777149107594293TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTAT181660.10307343934324416TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGTC180290.10229610469665026TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCTT178290.10116130959213364TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCCA177950.10096839442436582TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG176870.10035560506792686Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTTA176700.10025914748404292TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)