FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17624327
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG335354219.02791522195429Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2130871.2090504221806597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140010.6468388835499932No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGTG628900.3568363206152496Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCATGTGT345840.19622876947301307Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTC282480.1602784605619267Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG278220.15786134698930632Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT277030.15718614390211894Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT241870.13723644596471685No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT222630.126319717059267Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCAT202480.11488665638126211Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCATGTG184330.10458839080777382Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)