FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17782336
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG245720013.818207011722194Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1062310.5973962026136499No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGA892930.5021443751822033TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGT734560.41308408524054435TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG666550.3748382664684775Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTA490070.27559371277204525TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGC429570.2415711861478717TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGG396550.2230021972366285TruSeq Adapter, Index 7 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCT341370.19197140353213435TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCA326460.1835866783756645TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTA289690.16290885516953454TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTT285170.16036700689943098TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTG280870.1579488769079608TruSeq Adapter, Index 23 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC251040.14117380303690133TruSeq Adapter, Index 23 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT232740.1308826916778538Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGAA230620.1296904973564778TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCC229730.12919000068382466TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGGC229500.1290606588470716TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTC226850.1275704159453516TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGAC224230.1260970437179907TruSeq Adapter, Index 23 (97% over 37bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA203830.11462498515380655No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG200480.1127410931837077Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT197480.11105402574779827Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG197370.11099216660848159Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG185080.10408081367937261Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGAT182350.10254558231269503TruSeq Adapter, Index 23 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC179160.10075166727251132Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)