FastQCFastQC Report
Fri 3 May 2024
EGAF00007947789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17782336
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG274931715.460943938974047Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5166542.905433796774507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1022670.5751044182271666No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG481620.2708418061609003Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTGTGTGTAGATC477290.26840680549507107Illumina Single End PCR Primer 1 (97% over 39bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT403980.2271805009195642Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG373420.20999490730576678Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT328460.1847113899996041No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT249520.14031902220270723Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTGT217710.12243048382394756Illumina Single End PCR Primer 1 (97% over 39bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA208450.1172230690051071No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC204450.11497364575722784No Hit
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT189020.10629649557853367Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG182100.10240499335970259Illumina Single End PCR Primer 1 (100% over 32bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC181670.10216318036055556No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTC181160.10187637889645094Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)