FastQCFastQC Report
Sun 5 May 2024
EGAF00007947792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14647570
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1983671.3542655880804804No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1687181.1518497607452978Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA192840.13165323668021386No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA175550.11984923096459003No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)