FastQCFastQC Report
Sun 5 May 2024
EGAF00007947794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19002658
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG262071013.791281198661787Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990260.521116572218476No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG696440.3664960975459328Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAA541780.28510748338469283TruSeq Adapter, Index 1 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCT525410.2764928990460177TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAT423570.22290039635507833TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACT418960.22047441994693584TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA393530.20709208153932993TruSeq Adapter, Index 12 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATA389100.2047608287219609TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGC379580.19975100325438683TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCT357940.18836312267473318TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATT347050.1826323454329389TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTT342270.18011690785573262TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAC341660.17979590013144478TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT327910.17256007028069442Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTT322070.16948681600226664TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAC316570.16659248406196647TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAT315890.1662346393857112TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAT311740.16405073437621198TruSeq Adapter, Index 1 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA309960.16311402331189667TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTA308660.16242990848964392TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAT307940.1620510141265501TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG302780.1593356045243776Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGT302320.15909353312573432TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATT294340.15489412060144428TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTA294160.15479939701067083TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACA291000.15313647175042563TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATC288990.15207872498678868TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATA288060.15158931976779247TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAA287160.1511157018139252TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA286990.15102624064486136TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAA282830.14883707321365253TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGCT282180.14849501580252616TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG281700.1482424195604636Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT279200.14692681413305445TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACC277770.14617428782857642TruSeq Adapter, Index 1 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT274680.14454819952029868Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTC273820.14409563125326993TruSeq Adapter, Index 1 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTA273650.1440061700842061TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAA272800.143558864238887TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACA268220.14114867509587342TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATG264650.13926999054553316TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCT262260.13801227175693TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTC261690.13771231371948073TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACT245770.1293345383577392TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATG245740.1293187510926103TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAC235930.12415631539545678TruSeq Adapter, Index 1 (97% over 37bp)
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA232400.12229868053195506No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC231660.12190926132544194Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG226600.1192464759403658Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT225990.11892546821607797TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTG224610.11819925402014812TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCA222620.11715203209993046TruSeq Adapter, Index 1 (97% over 37bp)
GGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGCCAACCGAGCTTTGA217870.11465238178785306No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAGC215090.11318942855257406TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG209880.11044770684185339Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATC208810.10988462771892227TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG207530.10921103774008878Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAG204560.10764809849232669TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGA203540.10711133147794376TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGTT203180.10692188429639685TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCA202830.10673769953655957TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAC202310.10646405360765848TruSeq Adapter, Index 12 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAG202230.10642195423398139TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGCA198160.10428014859815928TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGA195130.10268563482013937TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAC193420.10178576070779151TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAGT191400.1007227515224449TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGT190560.10028070809883544TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)