FastQCFastQC Report
Mon 6 May 2024
EGAF00007947796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17480802
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG10070145.760685350706449Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2546591.456792428631135No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG370370.21187243010932794Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAC279420.1598439247810255TruSeq Adapter, Index 13 (97% over 36bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA279210.1597237929930217No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAA267390.15296208949680914TruSeq Adapter, Index 13 (97% over 36bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA241750.13829457023768132No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAT208600.1193309094170851TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAG187830.1074493035273782TruSeq Adapter, Index 13 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA184860.10575029681132479No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)